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by admin last modified Sep 18, 2017 12:39 PM

Sample data from Held et al. (2010) shows Hela Kyoto cells stably expressing different combinations of fluorescent markers. Full documentation of reagents and experimental details provided in the paper.

 

Chromatin + Microtubles

H2B-mCherry (red) + mEGFP-α-Tubulin (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and alpha-Tubulin.

Data acquired by Katja Beck (Kutay group, ETH Zurich) and Michael Held (Gerlich group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 20x dry objective (0.3225 µm / pixel), 1392x1040 pixel, 206 frames x 4.6 min = 15.8 h

H2b_aTub_MD20x_exp911_P0037_small.jpg
Overlay movies
  • Movie (MOV h264, 50% resolution, 18.6 MB)
  • Movie (MOV h264, 100% resolution, 138.4 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 3 positions, 899.6 MB)
  • Images (ZIP, extended set, 9 positions, 2.6 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 445.2 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 11.1 GB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.1 MB)
  • a-Tubulin (ZIP, annotated feature, libSVM model, w/ sample visualization, 2.7 MB)

 

Chromatin + Golgi aparatus

H2B-mCherry (red) + GalT-GFP (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and the Golgi aparatus (Galactosyl transferase, GalT).

Data acquired by Michael Schmitz (Gerlich group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 10x dry objective (0.645 µm / pixel), 1392x1040 pixel, 482 frames x 2.8 min = 22.5 h

H2b_GalT_MD10x_exp835_P0023_small.jpg
Overlay movies
  • Movie (MOV h264, 50% resolution, 30.4 MB)
  • Movie (MOV h264, 100% resolution, 252.5 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 1 position, 723.0 MB)
  • Images (ZIP, extended set, 6 positions, 4.0 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 18.6 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 18.6 MB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 2.8 MB)
  • GalT (ZIP, annotated feature, libSVM model, w/ sample visualization, 2.8 MB)


Chromatin + DNA replication factories

H2B-mCherry (red) + EGFP-PCNA (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and DNA replication factories (proliferation cell nuclear antigen, PCNA).

Data acquired by Michael Olma (Peter group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 10x dry objective (0.645 µm / pixel), 1392x1040 pixel, 482 frames x 5.9 min = 47.4 h

H2b_PCNA_MD10x_MOexp90_P0015_small.jpg

Overlay movies

  • Movie (MOV h264, 50% resolution, 12.9 MB)
  • Movie (MOV h264, 100% resolution, 163.9 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 1 position, 437.5 GB)
  • Images (ZIP, extended set, 4 positions, 3.4 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 848.7 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 11.4 GB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.9 MB)
  • PCNA (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.8 MB)
 

Chromatin + Nuclear import substrate

H2B-RFP (red) + IBB-mEGFP (green)

Human Hela Kyoto cells stably expressing fluorescent markers of chromatin (histone-2b, H2B) and the nuclear import substrate (importin-beta binding domain of importin-alpha, IBB).

Data acquired by Michael Schmitz (Gerlich group, ETH Zurich) at a Molecular Devices ImageXpress Micro with the PlateScanPackage.

Widefield epifluorescence, 10x dry objective (0.645 µm / pixel), 1392x1040 pixel, 744 frames x 3.7 min = 45.9 h

H2b_IBB_MD10x_exp665_P0028_small.jpg
Overlay movies
  • Movie (MOV h264, 50% resolution, 24.4 MB)
  • Movie (MOV h264, 100% resolution, 231.8 MB)
Raw data
  • Images (ZIP, reduced set for classifier retraining, 4 positions, 1.6 GB)
  • Images (ZIP, extended set, 6 positions, 2.5 GB)
Analysis data
  • Analysis (TAR.BZ2, features, tracking data, events, w/o visualization images, 490.4 MB)
  • Analysis (TAR.BZ2, features, tracking data, events, w/ visualization images, 11.7 GB)
Classifier data
  • H2B (ZIP, annotated feature, libSVM model, w/ sample visualization, 4.9 MB)

 

 

 

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